primary antibodies for v5  (Cell Signaling Technology Inc)

Journal: Frontiers in Oncology

Article Title: Focal adhesion kinase promotes ribosome biogenesis to drive advanced thyroid cancer cell growth and survival

doi: 10.3389/fonc.2025.1252544

Figure Lengend Snippet: pY397 FAK localizes to the nucleolus and influences nucleolar FAK accumulation (A) BCPAP, 8505C, and KTC2 cells were stained with antibodies against pY397 FAK, FBL, and DAPI prior to confocal imaging at 100X. (B) BCPAP, 8505C cells, and KTC2 cells were probed with antibodies against total FAK and DAPI prior to imaging at 100X confocal. Results shown are representative images from 20–30 images taken in at least two independent experiments. Scale bar represents 20μM. (C) FAK-KO cells transduced with EV, N-terminal NLS FAK, and N-terminal NLS-Y397F were probed with antibodies against V5, Fibrillarin, and DAPI prior to imaging at 100X on Olympus Confocal. At least 50 cells were imaged in three independent experiments. Scale bars represent 5μm. (D) Percent of co-localization between V5 and FBL in NLS versus NLS-Y397F KO-FAK transduced cells using Integrated Density Analysis by Image J. Results displayed as mean +/- SEM. *, p<0.05.

Article Snippet: 15,000-40,000 cells were grown on 8-well chamber slides for 48 hours prior to fixation with 4% paraformaldehyde (PFA) (Invitrogen) for 15 minutes, permeabilization with 0.5% Triton-X (Sigma) for 10 minutes, blocking with 5% normal goat serum (NGS) (Invitrogen), and incubation with primary antibodies against V5 (Cell Signaling), HA (Cell Signaling), Total FAK (BD Biosciences), pY397 FAK (Invitrogen), and fibrillarin (Invitrogen) overnight at 4°C.

Techniques: Staining, Imaging, Transduction